2011-04-26 21:58:43 UTC
Pro-oxidant iron in exhaled breath condensate: A potential excretory
mechanism. [JOURNAL ARTICLE]
Respir Med 2011 Apr 20.
Mumby S, Chung KF, McCreanor JE, Moloney ED, Griffiths MJ, Quinlan GJ
Pro-oxidant iron provides a potential measure of iron-catalysed
oxidative stress in biological fluids. This study aimed, to
investigate if the Bleomycin technique for measurement of pro-oxidant
iron in biological fluids could be utilised for determinations in
exhaled breath condensate (EBC). Secondly, to measure levels of pro-
oxidant iron in EBC from asthmatics after exposure to polluting city
Retrospective analysis of samples of EBC and bronchoalveolar lavage
fluid (BALF). Pro-oxidant iron levels were determined by the Bleomycin
method. Transferrin levels were determined by radial diffusion
immunoassay and lactoferrin by ELISA.
Patients undergoing surgery necessitating cardiopulmonary bypass,
normal healthy controls, "healthy" smokers, and asthmatics (mild and
Pro-oxidant iron was significantly decreased (p<0.05) post cardiac
surgery in both EBC and BALF. In smokers levels of pro-oxidant iron in
EBC were significantly (p<0.05) increased verses healthy controls. In
asthmatics with more severe disease, there were significant increases
in EBC pro-oxidant iron content post exposure to city environments
(p<0.001), with levels most elevated after exposure to the most
Similar patterns in the levels of pro-oxidant iron detectable in EBC
and paired BALF from patients undergoing cardiopulmonary bypass (pre
and post surgery) suggest a potential for EBC determinations.
Significantly elevated levels in EBC from smokers relative to control
subjects provide further support for this technique. In asthma disease
severity and environmental exposure influenced levels of pro-oxidant
iron measured in EBC indicating a potential for enhanced iron-
catalysed oxidative stress.
Respiratory medicine [Respir Med]
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